HSD17B2 antibody (C-Term) (APC)

Catalog No. ABIN7998814

The Rabbit Polyclonal anti-HSD17B2 antibody is suitable to detect HSD17B2 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for HSD17B2 antibody (C-Term) (APC) (ABIN7998814)

Target: HSD17B2 (Hydroxysteroid (17-Beta) Dehydrogenase 2 (HSD17B2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD17B2 antibody is conjugated to APC

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HSD17B2 Antibody (APC)

Binding Specificity: C-Term

Purpose: Anti-HSD17B2 Antibody APC Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HSD17B2.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Applications are recommended based on the unconjugated antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for HSD17B2

Target: HSD17B2 (Hydroxysteroid (17-Beta) Dehydrogenase 2 (HSD17B2))

Alternative Name: HSD17B2

Background:

Background: HSD17B2 (17-BETA-HYDROXYSTEROID DEHYDROGENASE II), also called 17-BETA-HSD II, is an anzyme which have 387 amino acids with a predicted molecular weight of 42,782 and associate with the membranes of the endoplasmic reticulum. Its Cytogenetic location is 16q23.3. The type 2 enzyme was capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. HSD17B2 mRNA was detected in 18 of 42 (43 % ) adenomas but not in prolactinomas. In the human endometrium, inactivation of 17-beta-estradiol to estrone is catalyzed by HSD17B2. And HSD17B2 activity distinguishing between disease-free and diseased endometria. HSD17B2 efficiently catalyzes the oxidative metabolism of androgens and estrogens, and it is expressed in a large series of human peripheral tissues. The previous paradigm that HSD17B2 activity in the endometrium is elevated during the secretory phase is confined to diseased endometrium but not to disease-free endometrium.

Gene Full Name: hydroxysteroid 17-beta dehydrogenase 2

Sequence Similarities: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Gene ID: 3294

UniProt: P37059

Pathways: Steroid Hormone Biosynthesis