IST1 antibody (AA 31-331)

Catalog No. ABIN7999312

The Rabbit Polyclonal anti-IST1 antibody is suitable to detect IST1 in samples from Human, Mouse and Rat. It has been validated for ELISA, WB, IHC, FACS, IF and ICC.

Quick Overview for IST1 antibody (AA 31-331) (ABIN7999312)

Target: IST1 (Increased Sodium Tolerance 1 Homolog (IST1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IST1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-IST1 Antibody

Binding Specificity: AA 31-331

Purpose: Anti-hIST1/IST1 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human IST1 shares 94%,93.1% amino acid (aa) sequence identity with mouse,rat IST1,respectively.

Characteristics: Anti-hIST1/IST1 Antibody. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human hIST1/IST1 recombinant protein (Position: E31-D331). Human IST1 shares 94% and 93.1% amino acid (aa) sequence identity with mouse and rat IST1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for IST1

Target: IST1 (Increased Sodium Tolerance 1 Homolog (IST1))

Alternative Name: IST1

Background:

Background: IST1 homolog is a protein that in humans is encoded by the KIAA0174 gene. This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Gene Full Name: IST1 factor associated with ESCRT-III

Molecular Weight: 40 kDa

Gene ID: 9798

UniProt: P53990

Pathways: Regulation of Cell Size