Indian Hedgehog antibody (AA 227-360)

Catalog No. ABIN7999324

The Rabbit Polyclonal anti-Indian Hedgehog antibody is suitable to detect Indian Hedgehog in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, FACS and IF.

Quick Overview for Indian Hedgehog antibody (AA 227-360) (ABIN7999324)

Target: Indian Hedgehog (IHH)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Indian Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-Indian Hedgehog Antibody

Binding Specificity: AA 227-360

Purpose: Anti-IHH Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human IHH shares 94.8% amino acid (aa) sequence identity with mouse IHH.

Characteristics: Anti-IHH Antibody. Tested in WB, IHC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IHH recombinant protein (Position: D227-A360). Human IHH shares 94.8% amino acid (aa) sequence identity with mouse IHH.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Indian Hedgehog

Target: Indian Hedgehog (IHH)

Alternative Name: IHH

Background:

Background: This gene encodes a member of the hedgehog family of proteins. The encoded preproprotein is proteolytically processed to generate multiple protein products, including an N-terminal fragment that is involved in signaling. Hedgehog family proteins are essential secreted signaling molecules that regulate a variety of developmental processes including growth, patterning and morphogenesis. The protein encoded by this gene specifically plays a role in bone growth and differentiation. Mutations in this gene are the cause of brachydactyly type A1, which is characterized by shortening or malformation of the fingers and toes. Mutations in this gene are also the cause of acrocapitofemoral dysplasia.

Gene Full Name: Indian hedgehog signaling molecule

Molecular Weight: 45 kDa

Gene ID: 3549

UniProt: Q14623

Pathways: Hedgehog Signaling