IGFBP5 antibody (N-Term) (Biotin)

Catalog No. ABIN8000209

The Biotin-conjugated Rabbit Polyclonal anti-IGFBP5 antibody (ABIN8000209) specifically detects IGFBP5 in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for IGFBP5 antibody (N-Term) (Biotin) (ABIN8000209)

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGFBP5 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-IGFBP5 Antibody (Biotin)

Binding Specificity: N-Term

Purpose: Anti-IGFBP5 Antibody Biotin Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse,rat sequences by two amino acids.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human IGFBP5, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for IGFBP5

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Alternative Name: IGFBP5

Background:

Background: Insulin-like growth factor-binding protein 5?is a?protein?that in humans is encoded by the?IGFBP5?gene. The expression of?IGFBP5?by stable transfection and adenovirus-mediated infection is inhibitory to growth in 2 human breast cancer cell lines.?IGFBP5?expression leads to G2/M cell cycle arrest and apoptosis. Stable expression of?IGFBP5?in the breast cancer cell lines also inhibits the formation and growth of tumors following injection in athymic mice. It is concluded that IGFBP5?is a growth inhibitor and proapoptotic agent in breast cancer cells. Additionally, IGFBP-5 is expressed by fibroblasts, myoblasts and osteoblasts, making it the predominant IGFBP found in bone extracts. It has a strong affinity for hydroxyapatite, allowing it to bind to bone cells. When bound to extracellular matrix, IGFBP-5 is protected from proteolysis and potentiates IGF activity, but when it is soluble, IGFBP-5 is cleaved to a biologically inactive 21? kDa fragment (1,?2).

Gene Full Name: insulin like growth factor binding protein 5

Gene ID: 3488

UniProt: P24593

Pathways: WNT Signaling, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding