IRF3 antibody (AA 1-419) (PE)

Catalog No. ABIN8001195

The Mouse Monoclonal anti-IRF3 antibody is suitable to detect IRF3 in samples from Mouse and Rat. It has been validated for FACS.

Quick Overview for IRF3 antibody (AA 1-419) (PE) (ABIN8001195)

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This IRF3 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 3B4

Product Details anti-IRF3 Antibody (PE)

Binding Specificity: AA 1-419

Purpose: Anti-IRF3 Antibody (monoclonal, 3B4) PE Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse IRF3 recombinant protein (Position: M1-I419).

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Alternative Name: Irf3

Background:

Background: IRF3 (interferon regulatory factor 3) is a member of the interferon regulatory transcription factor (IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. IRF3 plays an important role in the innate immune system's response to viral infection. Aggregated MAVS have been found to activate IRF3 dimerization. Although IRF3 increased transcriptional activity from an ISRE-containing promoter, expression of IRF3 as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase (CAT) < a href=https://www.bosterbio.com/cells/reporter-cell-lines style=color:#ea8d28>reporter gene containing repeats of the Gal4-binding sites. Translocation of IRF3 was accompanied by an increase in serine and threonine phosphorylation. The transcriptional activators CREBBP and EP300 coimmunoprecipitated with IRF3 only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1.

Gene Full Name: interferon regulatory factor 3

Gene ID: 54131

UniProt: P70671

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades