IRF3 antibody (AA 1-427) (Biotin)

Catalog No. ABIN8001209

This Rabbit Polyclonal antibody specifically detects IRF3 in WB, ELISA and IHC. It exhibits reactivity toward Human and Mouse.

Quick Overview for IRF3 antibody (AA 1-427) (Biotin) (ABIN8001209)

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF3 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-IRF3 Antibody (Biotin)

Binding Specificity: AA 1-427

Purpose: Anti-IRF3 Antibody Biotin Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IRF3 recombinant protein (Position: M1-S427).

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Alternative Name: IRF3

Background:

Background: IRF3 (interferon regulatory factor 3)is a member of the interferon regulatory transcription factor (IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex withCREBBP. IRF3 plays an important role in theinnate immune system's response toviral infection. AggregatedMAVShave been found to activate IRF3 dimerization. AlthoughIRF3increased transcriptional activity from an ISRE-containing promoter, expression ofIRF3as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase (CAT) < a href=https://www.bosterbio.com/cells/reporter-cell-lines style=color:#ea8d28>reporter gene containing repeats of the Gal4-binding sites.Translocation of IRF3 was accompanied by an increase in serine and threonine phosphorylation.The transcriptional activators CREBBP and EP300 coimmunoprecipitated withIRF3only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1.

Gene Full Name: interferon regulatory factor 3

Gene ID: 3661

UniProt: Q14653

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades