IL-18 antibody (AA 37-193) (Cy3)

Catalog No. ABIN8002135

The Cy3-conjugated Rabbit Polyclonal anti-IL-18 antibody (ABIN8002135) specifically detects IL-18 in FACS. The antibody is reactive with Human samples.

Quick Overview for IL-18 antibody (AA 37-193) (Cy3) (ABIN8002135)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-18 antibody is conjugated to Cy3

Application: Flow Cytometry (FACS)

Product Details anti-IL-18 Antibody (Cy3)

Binding Specificity: AA 37-193

Purpose: Anti-IL18 Antibody Cy3 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human IL-18 shares 66%,64% amino acid (aa) sequences identity with mouse,rat IL-18,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IL-18 recombinant protein (Position: Y37-D193). Human IL-18 shares 66% and 64% amino acid (aa) sequences identity with mouse and rat IL-18, respectively.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, 1-3 μg/1x10⁶ cells

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: IL18

Background:

Background: Interleukin-18, also known as IL18, is a protein which in humans is encoded by the IL18 gene. IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. It is mapped to 11q22.2-q22.3. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12, it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-γ (IFN-γ) or type II interferon that plays an important role in activating the macrophages or other cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.

Gene Full Name: interleukin 18

Sequence Similarities: Belongs to the IL-1 family.

Gene ID: 3606

UniProt: Q14116

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome