IL-18 antibody (AA 37-193)

Catalog No. ABIN8002144

The Rabbit Polyclonal anti-IL-18 antibody (ABIN8002144) specifically detects IL-18 in WB, IHC, ICC and IF. The antibody is reactive with Human samples.

Quick Overview for IL-18 antibody (AA 37-193) (ABIN8002144)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-IL-18 Antibody

Binding Specificity: AA 37-193

Purpose: Anti-IL18 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human IL-18 shares 66%,64% amino acid (aa) sequences identity with mouse,rat IL-18,respectively.

Characteristics: Anti-IL18 Antibody. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IL-18 recombinant protein (Position: Y37-D193). Human IL-18 shares 66% and 64% amino acid (aa) sequences identity with mouse and rat IL-18, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: IL18

Background:

Background: Interleukin-18, also known as IL18, is a protein which in humans is encoded by the IL18 gene. IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. It is mapped to 11q22.2-q22.3. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12, it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-γ (IFN-γ) or type II interferon that plays an important role in activating the macrophages or other cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.

Gene Full Name: interleukin 18

Sequence Similarities: Belongs to the IL-1 family.

Molecular Weight: 22 kDa

Gene ID: 3606

UniProt: Q14116

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome