IRAK4 antibody (AA 15-460) (HRP)

Catalog No. ABIN8002987

The HRP-conjugated Rabbit Polyclonal anti-IRAK4 antibody (ABIN8002987) specifically detects IRAK4 in WB, ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for IRAK4 antibody (AA 15-460) (HRP) (ABIN8002987)

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRAK4 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-IRAK4 Antibody (HRP)

Binding Specificity: AA 15-460

Purpose: Anti-IRAK4 Antibody HRP Conjugated

Predicted Reactivity: Human IRAK4 shares 84% amino acid (aa) sequence identity with mouse IRAK4.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IRAK4 recombinant protein (Position: N15-S460). Human IRAK4 shares 84% amino acid (aa) sequence identity with mouse IRAK4.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for IRAK4

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Alternative Name: IRAK4

Background:

Background: IRAK-4(interleukin-1 receptor-associated kinase 4), also called REN64, in the IRAK family, is a protein kinase involved in signaling innate immune responses from Toll-like receptors. It also supports signaling from T-cell receptors. Scott(2002) mapped the REN64/IRAK4 gene to chromosome 12 based on similarity between the REN64 sequence(GenBank AF155118) and a chromosome 12 clone(GenBank AC093012). Functional analysis by Li et al.(2002) determined that IRAK4, like IRAK1 and Pelle, has auto- and cross-phosphorylation kinase activity. Precipitation and binding analyses showed weak interaction between IRAK4 and IRAK1, but IRAK4 did not interact with other IRAK family members. Overexpressed IRAK4 interacted with MYD88 and TRAF6 and activated mitogen-activated protein kinase(MAPK) and nuclear factor kappa-B(NFKB) pathways.

Gene Full Name: interleukin 1 receptor associated kinase 4

Gene ID: 51135

UniProt: Q9NWZ3

Pathways: NF-kappaB Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades