IRAK4 antibody (N-Term) (HRP)

Catalog No. ABIN8002998

The Rabbit Polyclonal anti-IRAK4 antibody is suitable to detect IRAK4 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for IRAK4 antibody (N-Term) (HRP) (ABIN8002998)

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRAK4 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-IRAK4 Antibody (HRP)

Binding Specificity: N-Term

Purpose: Anti-IRAK4 Antibody HRP Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat,mouse sequences.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human IRAK4, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for IRAK4

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Alternative Name: IRAK4

Background:

Background: IRAK-4 (interleukin-1 receptor-associated kinase 4), also called REN64, in the IRAK family, is a protein kinase involved in signaling innate immune responses from Toll-like receptors. It also supports signaling from T-cell receptors. Scott (2002) mapped the REN64/IRAK4 gene to chromosome 12 based on similarity between the REN64 sequence (GenBank AF155118) and a chromosome 12 clone (GenBank AC093012). Functional analysis by Li et al. (2002) determined that IRAK4, like IRAK1 and Pelle, has auto- and cross-phosphorylation kinase activity. Precipitation and binding analyses showed weak interaction between IRAK4 and IRAK1, but IRAK4 did not interact with other IRAK family members. Overexpressed IRAK4 interacted with MYD88 and TRAF6 and activated mitogen-activated protein kinase (MAPK) and nuclear factor kappa-B (NFKB) pathways.

Gene Full Name: interleukin 1 receptor associated kinase 4

Sequence Similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

Gene ID: 51135

UniProt: Q9NWZ3

Pathways: NF-kappaB Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades