CYBB antibody (AA 501-570)

Catalog No. ABIN750688

This Rabbit Polyclonal antibody specifically detects CYBB in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat, Rabbit and Chicken and has been mentioned in 6+ publications.

Quick Overview for CYBB antibody (AA 501-570) (ABIN750688)

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Reactivity: Human, Mouse, Rat, Rabbit, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYBB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CYBB Antibody

Binding Specificity: AA 501-570

Cross-Reactivity: Chicken, Human, Mouse, Rabbit, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NOX2/gp91phox

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CYBB antibody (ABIN750688)

Ye, Wu, Liu, Chen, Zhang, Lu, Zhou, Li, Zhang, Xia, Zhuang, Hang et al.: "Inhibition of leukotriene B4 synthesis protects against early brain injury possibly via reducing the neutrophil-generated inflammatory response and oxidative stress after subarachnoid hemorrhage in ..." in: Behavioural brain research, Vol. 339, pp. 19-27, (2017) (PubMed).

Wakatsuki, Furuno, Ohshima, Araki: "Oxidative stress-dependent phosphorylation activates ZNRF1 to induce neuronal/axonal degeneration." in: The Journal of cell biology, Vol. 211, Issue 4, pp. 881-96, (2015) (PubMed).

Kashiwabara, Ambe, Nakagawa, Watanabe: "Immunohistochemical localization of Nox in mouse circumvallate papillae." in: Tissue & cell, Vol. 47, Issue 6, pp. 550-8, (2015) (PubMed).

Wang, Li, Cheng, Zhang, Narsinh, Li, Li, Qin, Zhang, Li, Su, Chen, Cao: "Activation of Liver X Receptor Improves Viability of Adipose-Derived Mesenchymal Stem Cells to Attenuate Myocardial Ischemia Injury Through TLR4/NF-?B and Keap-1/Nrf-2 Signaling Pathways." in: Antioxidants & redox signaling, (2014) (PubMed).

Dalaklioglu, Tasatargil, Kale, Tanriover, Dilmac, Erin: "Metastatic breast carcinoma induces vascular endothelial dysfunction in Balb-c mice: Role of the tumor necrosis factor-? and NADPH oxidase." in: Vascular pharmacology, Vol. 59, Issue 3-4, pp. 103-11, (2013) (PubMed).

Lagadinou, Sach, Callahan, Rossi, Neering, Minhajuddin, Ashton, Pei, Grose, ODwyer, Liesveld, Brookes, Becker, Jordan: "BCL-2 inhibition targets oxidative phosphorylation and selectively eradicates quiescent human leukemia stem cells." in: Cell stem cell, Vol. 12, Issue 3, pp. 329-41, (2013) (PubMed).

Target Details for CYBB

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Alternative Name: NOX2/gp91phox

Background:

Synonyms: CGD, NOX2, IMD34, AMCBX2, GP91-1, GP91PHOX, p91-PHOX, GP91-PHOX, Cytochrome b-245 heavy chain, CGD91-phox, Cytochrome b(558) subunit beta, Cytochrome b558 subunit beta, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, Neutrophil cytochrome b 91 kDa polypeptide, Superoxide-generating NADPH oxidase heavy chain subunit, p22 phagocyte B-cytochrome, CYBB

Background: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.

Gene ID: 1536

UniProt: P04839