KEAP1 antibody (AA 25-205) (Fluoro488)

Catalog No. ABIN8004050

This Rabbit Polyclonal antibody specifically detects KEAP1 in FACS. It exhibits reactivity toward Human.

Quick Overview for KEAP1 antibody (AA 25-205) (Fluoro488) (ABIN8004050)

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KEAP1 antibody is conjugated to Fluoro488

Application: Flow Cytometry (FACS)

Product Details anti-KEAP1 Antibody (Fluoro488)

Binding Specificity: AA 25-205

Purpose: Anti-Keap1 Antibody Fluoro488 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human Keap1 shares 96.7%,97.2% amino acid (aa) sequence identity with mouse,rat Keap1,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Keap1 recombinant protein (Position: E25-E205). Human Keap1 shares 96.7% and 97.2% amino acid (aa) sequence identity with mouse and rat Keap1, respectively.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Alternative Name: KEAP1

Background:

Background: KEAP1 (KELCH-LIKE ECH-ASSOCIATED PROTEIN 1), is a protein that in humans is encoded by the Keap1 gene. The KIAA0132 gene is mapped on 19p13.2. Keap1 contains a central BTB/POZ domain and a C-terminal double glycine repeat (DGR), or Kelch, module. Keap1 has been shown to interact with Nrf2, a master regulator of the antioxidant response, which is important for the amelioration of oxidative stress. In the presence of the electrophilic agent diethylmalate, Nrf2 activity is released from Keap1 and Nrf2 translocate to the nucleus. Under quiescent conditions, Nrf2 is anchored in the cytoplasm through binding to Keap1, which, in turn, facilitates the ubiquitination and subsequent proteolysis of Nrf2. Because Nrf2 activation leads to a coordinated antioxidant and anti-inflammatory response, and Keap1 represses Nrf2 activation, Keap1 has become a very attractive drug target.

Gene Full Name: kelch like ECH associated protein 1

Gene ID: 9817

UniProt: Q14145

Pathways: Maintenance of Protein Location