LIF antibody (AA 23-202)

Catalog No. ABIN8005875

The Rabbit Polyclonal anti-LIF antibody is suitable to detect LIF in samples from Human. It has been validated for WB and IHC.

Quick Overview for LIF antibody (AA 23-202) (ABIN8005875)

Target: LIF (Leukemia Inhibitory Factor (LIF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-LIF Antibody

Binding Specificity: AA 23-202

Purpose: Anti-LIF Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human LIF shares 78%,82% amino acid (aa) sequences identity with mouse,rat LIF,respectively.

Characteristics: Anti-LIF Antibody. Tested in IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human LIF recombinant protein (Position: S23-F202). Human LIF shares 78% and 82% amino acid (aa) sequences identity with mouse and rat LIF, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, HumanWestern blot, 0.1-0.5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for LIF

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: LIF

Background:

Background: LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. This gene is mapped to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. LIF could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface.

Gene Full Name: LIF interleukin 6 family cytokine

Sequence Similarities: Belongs to the LIF/OSM family.

Molecular Weight: 36 kDa

Gene ID: 3976

UniProt: P15018

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding