LIG3 antibody (AA 297-963)

Catalog No. ABIN8005985

This Rabbit Polyclonal antibody specifically detects LIG3 in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for LIG3 antibody (AA 297-963) (ABIN8005985)

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-LIG3 Antibody

Binding Specificity: AA 297-963

Purpose: Anti-DNA Ligase III/LIG3 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-DNA Ligase III/LIG3 Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DNA Ligase III/LIG3 recombinant protein (Position: D297-D963).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for LIG3

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Alternative Name: LIG3

Background:

Background: DNA ligase 3 is an enzyme that, in humans, is encoded by the LIG3 gene. This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Gene Full Name: DNA ligase 3

Molecular Weight: 113 kDa

Gene ID: 3980

UniProt: P49916

Pathways: DNA Damage Repair