Kdm6b antibody (AA 1127-1643)

Catalog No. ABIN8006998

The Rabbit Polyclonal anti-Kdm6b antibody (ABIN8006998) specifically detects Kdm6b in WB, ELISA and FACS. The antibody is reactive with Human and Mouse samples.

Quick Overview for Kdm6b antibody (AA 1127-1643) (ABIN8006998)

Target: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Kdm6b antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-Kdm6b Antibody

Binding Specificity: AA 1127-1643

Purpose: Anti-KDM6B/JMJD3 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-KDM6B/JMJD3.Antibody-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human KDM6B/JMJD3 recombinant protein (Position: R1127-R1643).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Kdm6b

Target: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Alternative Name: KDM6B

Background:

Background: Lysine demethylase 6B is a protein that in humans is encoded by the KDM6B gene. It is mapped to 17p13.1. The protein encoded by this gene is a lysine-specific demethylase that specifically demethylates di- or tri-methylated lysine 27 of histone H3 (H3K27me2 or H3K27me3). H3K27 trimethylation is a repressive epigenetic mark controlling chromatin organization and gene silencing. This protein can also demethylate non-histone proteins such as retinoblastoma protein. Through its demethylation actvity this gene influences cellular differentiation and development, tumorigenesis, inflammatory diseases, and neurodegenerative diseases. This protein has two classical nuclear localization signals at its N-terminus. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Gene Full Name: lysine demethylase 6B

Molecular Weight: 177 kDa

Gene ID: 23135

UniProt: O15054

Pathways: Warburg Effect