Macro H2A.2 (AA 181-340) antibody (Fluoro550)

Catalog No. ABIN8007200

This Rabbit Polyclonal antibody specifically detects Macro H2A.2 in WB, ELISA, IHC, FACS, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Macro H2A.2 (AA 181-340) antibody (Fluoro550) (ABIN8007200)

Target: Macro H2A.2

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Fluoro550

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details

Binding Specificity: AA 181-340

Purpose: Anti-H2AFY2/MACROH2A2 Antibody Fluoro550 Conjugated

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human MACROH2A2 shares 99.4% amino acid (aa) sequence identity with mouse MACROH2A2.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human H2AFY2/MACROH2A2 recombinant protein (Position: D181-D340). Human MACROH2A2 shares 99.4% amino acid (aa) sequence identity with mouse MACROH2A2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Comment:

Applications are recommended based on the unconjugated antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details

Target: Macro H2A.2

Alternative Name: MACROH2A2

Background:

Background: Core histone macro-H2A.2 is a protein that in humans is encoded by the H2AFY2 gene. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and may participate in stable X chromosome inactivation.

Gene Full Name: macroH2A.2 histone

Gene ID: 55506

UniProt: Q9P0M6