MMP12 antibody (AA 237-442)

Catalog No. ABIN8008065

The Rabbit Polyclonal anti-MMP12 antibody is suitable to detect MMP12 in samples from Mouse. It has been validated for WB and ELISA.

Quick Overview for MMP12 antibody (AA 237-442) (ABIN8008065)

Target: MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Carrier-free

Product Details anti-MMP12 Antibody

Binding Specificity: AA 237-442

Purpose: Anti-Mmp12 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Mouse Mmp12 shares 62.7%,90.3% amino acid (aa) sequence identity with mouse,rat Mmp12,respectively.

Characteristics: Anti-Mmp12 Antibody. Tested in WB, ELISA applications. This antibody reacts with Mouse.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Mmp12 recombinant protein (Position: K237-H442). Mouse Mmp12 shares 62.7% and 90.3% amino acid (aa) sequence identity with mouse and rat Mmp12, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MMP12

Target: MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

Alternative Name: Mmp12

Background:

Background: Matrix metalloproteinase-12 (MMP-12) also known as macrophage metalloelastase (MME) or macrophage elastase (ME) is an enzyme that in humans is encoded by the MMP12 gene. This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme. Mice lacking the encoded protein have a diminished capacity to degrade extracellular matrix components, do not develop emphysema in response to long-term exposure to cigarette smoke, and exhibit impaired clearance and increased mortality upon bacterial infection. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. Alternate splicing generates multiple transcript variants encoding distinct isoforms.

Gene Full Name: matrix metallopeptidase 12

Molecular Weight: 45 kDa

Gene ID: 17381

UniProt: P34960