MED12 antibody (AA 752-1974) (APC)

Catalog No. ABIN8008535

This Rabbit Polyclonal antibody specifically detects MED12 in FACS. It exhibits reactivity toward Human.

Quick Overview for MED12 antibody (AA 752-1974) (APC) (ABIN8008535)

Target: MED12 (Mediator Complex Subunit 12 (MED12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED12 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Product Details anti-MED12 Antibody (APC)

Binding Specificity: AA 752-1974

Purpose: Anti-MED12 Antibody APC Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MED12 recombinant protein (Position: Q752-Q1974).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for MED12

Target: MED12 (Mediator Complex Subunit 12 (MED12))

Alternative Name: MED12

Background:

Background: Mediator of RNA polymerase II transcription, subunit 12 homolog (S. cerevisiae), also known as MED12, is a human gene found on the X chromosome. The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome.

Gene Full Name: mediator complex subunit 12

Gene ID: 9968

UniProt: Q93074

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Tube Formation