MED12 antibody (AA 752-1974)

Catalog No. ABIN8008538

This Rabbit Polyclonal antibody specifically detects MED12 in ELISA and WB. It exhibits reactivity toward Human.

Quick Overview for MED12 antibody (AA 752-1974) (ABIN8008538)

Target: MED12 (Mediator Complex Subunit 12 (MED12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Grade: Carrier-free

Product Details anti-MED12 Antibody

Binding Specificity: AA 752-1974

Purpose: Anti-MED12 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MED12 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MED12 recombinant protein (Position: Q752-Q1974).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MED12

Target: MED12 (Mediator Complex Subunit 12 (MED12))

Alternative Name: MED12

Background:

Background: Mediator of RNA polymerase II transcription, subunit 12 homolog (S. cerevisiae), also known as MED12, is a human gene found on the X chromosome. The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome.

Gene Full Name: mediator complex subunit 12

Molecular Weight: 260 kDa

Gene ID: 9968

UniProt: Q93074

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Tube Formation