MED4 antibody (AA 27-247) (HRP)

Catalog No. ABIN8008677

The Rabbit Polyclonal anti-MED4 antibody is suitable to detect MED4 in samples from Human, Rat and Mouse. It has been validated for WB, ELISA and IHC.

Quick Overview for MED4 antibody (AA 27-247) (HRP) (ABIN8008677)

Target: MED4 (Mediator Complex Subunit 4 (MED4))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED4 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-MED4 Antibody (HRP)

Binding Specificity: AA 27-247

Purpose: Anti-MED4 Antibody HRP Conjugated

Predicted Reactivity: Human MED4 shares 96.8%,96.4% amino acid (aa) sequence identity with mouse,rat MED4,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MED4 recombinant protein (Position: R27-H247). Human MED4 shares 96.8% and 96.4% amino acid (aa) sequence identity with mouse and rat MED4, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MED4

Target: MED4 (Mediator Complex Subunit 4 (MED4))

Alternative Name: MED4

Background:

Background: Mediator of RNA polymerase II transcription subunit 4 also known as mediator complex subunit 4 (MED4), a component of Mediator or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36) is a protein that in humans is encoded by the MED4 gene. It is mapped to 13q14.2. This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Gene Full Name: mediator complex subunit 4

Gene ID: 29079

UniProt: Q9NPJ6

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha