MGEA5 antibody (C-Term) (Cy3)

Catalog No. ABIN8009134

The Cy3-conjugated Rabbit Polyclonal anti-MGEA5 antibody (ABIN8009134) specifically detects MGEA5 in WB, IHC, ICC, IF and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MGEA5 antibody (C-Term) (Cy3) (ABIN8009134)

Target: MGEA5 (Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MGEA5 antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-MGEA5 Antibody (Cy3)

Binding Specificity: C-Term

Purpose: Anti-MGEA5/OGA Antibody Cy3 Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: identical to the related mouse,rat sequences.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at C-terminus of human OGA, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Applications are recommended based on the unconjugated antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for MGEA5

Target: MGEA5 (Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5))

Alternative Name: OGA

Background:

Background: Protein O-GlcNAcase (EC 3.2.1.169, OGA, glycoside hydrolase O-GlcNAcase, O-GlcNAcase, BtGH84, O-GlcNAc hydrolase) is an enzyme with systematic name (protein)-3-O- (N-acetyl-D-glucosaminyl)-L-serine/threonine N-acetylglucosaminyl hydrolase. The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications.

Gene Full Name: O-GlcNAcase

Gene ID: 10724

UniProt: O60502

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Carbohydrate Metabolic Process