Midkine antibody (AA 21-143)

Catalog No. ABIN8010189

The Rabbit Polyclonal anti-Midkine antibody is suitable to detect Midkine in samples from Human. It has been validated for WB.

Quick Overview for Midkine antibody (AA 21-143) (ABIN8010189)

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Midkine antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-Midkine Antibody

Binding Specificity: AA 21-143

Purpose: Anti-Midkine/MDK Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human Midkine shares 87%,89% amino acid (aa) sequence identity with mouse,rat Midkine,respectively.

Characteristics: Anti-Midkine/MDK Antibody. Tested in WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Midkine recombinant protein (Position: V21-D143). Human Midkine shares 87% and 89% amino acid (aa) sequence identity with mouse and rat Midkine, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Midkine

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Alternative Name: MDK

Background:

Background: Midkine (MK or MDK), also known as neurite growth-promoting factor 2 (NEGF2), is a protein that in humans is encoded by the MDK gene. Midkine is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophin (NEGF1, 46% homologous with MK). It is a nonglycosylated protein, composed of two domains held by disulfide bridges. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair. Midkine is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. A molecular complex containing receptor-type tyrosine phosphatase zeta (PTPζ), low density lipoprotein receptor-related protein (LRP1), anaplastic leukemia kinase (ALK) and syndecans is considered to be its receptor.

Gene Full Name: midkine

Sequence Similarities: Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

Molecular Weight: 16 kDa

Gene ID: 4192

UniProt: P21741

Pathways: RTK Signaling, M Phase, Skeletal Muscle Fiber Development