MFGE8 antibody (AA 23-463) (Biotin)

Catalog No. ABIN8010212

This Rabbit Polyclonal antibody specifically detects MFGE8 in WB, IHC and ELISA. It exhibits reactivity toward Mouse and Rat.

Quick Overview for MFGE8 antibody (AA 23-463) (Biotin) (ABIN8010212)

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MFGE8 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-MFGE8 Antibody (Biotin)

Binding Specificity: AA 23-463

Purpose: Anti-Milk Fat Globule 1/Mfge8 Antibody Biotin Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Mouse?Mfge8 shares?86.2%?amino?acid?(aa)?sequence?identity?with rat Mfge8.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Milk Fat Globule 1/Mfge8 recombinant protein (Position: A23-C463). Mouse?Mfge8 shares?86.2%?amino?acid?(aa)?sequence?identity?with rat Mfge8.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MFGE8

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Alternative Name: Mfge8

Background:

Background: MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, Collins et al. (1997) mapped the MFGE8 gene to chromosome 15q25. Hanayama et al. (2002) found that MFGE8 is a factor that links apoptotic cells to phagocytes. MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. Bu et al. (2007) showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.

Gene Full Name: milk fat globule EGF and factor V/VIII domain containing

Gene ID: 17304

UniProt: P21956

Pathways: SARS-CoV-2 Protein Interactome