MCM7 antibody (AA 526-719) (Biotin)

Catalog No. ABIN8010443

This Mouse Monoclonal antibody specifically detects MCM7 in WB, IHC and ELISA. It exhibits reactivity toward Human and Rat.

Quick Overview for MCM7 antibody (AA 526-719) (Biotin) (ABIN8010443)

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCM7 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 3H11

Product Details anti-MCM7 Antibody (Biotin)

Binding Specificity: AA 526-719

Purpose: Anti-MCM7 Antibody (monoclonal, 3H11) Biotin Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human MCM7 shares 94% amino acid (aa) sequence identity with MCM7.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MCM7 recombinant protein (Position: D526-V719). Human MCM7 shares 94% amino acid (aa) sequence identity with MCM7.

Isotype: IgG1

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MCM7

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Alternative Name: MCM7

Background:

Background: MCM7(Minichromosome Maintenance, s. Cerevisiae, homolog of, 7), also called CDC47, FORMERLY, is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The MCM7 gene is mapped to 7q22.1. MCM7 plays a pivotal role in the G1/S phase transition, orchestrating the correct assembly of replication forks on chromosomal DNA and ensuring that all the genome is replicated once and not more than once at each cell cycle. The MCM7 gene contains 15 exons. The miRNAs MIR106B, MIR93, and MIR25 are clustered in a 5-prime to 3-prime orientation within intron 13. It has been found that MCM7 and the precursors of microRNAs (miRNAs) MIR106B, MIR93, and MIR25, all of which arise from intron 13 of the MCM7 gene, were overexpressed with almost perfect correlation in 5 of 10 human gastric tumors.

Gene Full Name: minichromosome maintenance complex component 7

Gene ID: 4176

UniProt: P33993

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA