MLKL antibody (AA 210-464) (Fluoro594)

Catalog No. ABIN8011459

The Fluoro594-conjugated Rabbit Polyclonal anti-MLKL antibody (ABIN8011459) specifically detects MLKL in FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MLKL antibody (AA 210-464) (Fluoro594) (ABIN8011459)

Target: MLKL (Mixed Lineage Kinase Domain-Like (MLKL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLKL antibody is conjugated to Fluoro594

Application: Flow Cytometry (FACS)

Product Details anti-MLKL Antibody (Fluoro594)

Binding Specificity: AA 210-464

Purpose: Anti-MLKL Antibody Fluoro594 Conjugated

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human MLKL shares 69.7% amino acid (aa) sequence identity with mouse MLKL.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MLKL recombinant protein (Position: R210-K464). Human MLKL shares 69.7% amino acid (aa) sequence identity with mouse MLKL.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for MLKL

Target: MLKL (Mixed Lineage Kinase Domain-Like (MLKL))

Alternative Name: MLKL

Background:

Background: Mixed lineage kinase domain like pseudokinase (MLKL) is a protein that in humans is encoded by the MLKL gene. This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain, however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.

Gene Full Name: mixed lineage kinase domain like pseudokinase

Gene ID: 197259

UniProt: Q8NB16