MPL antibody (AA 26-595) (Fluoro550)

Catalog No. ABIN8012514

The Rabbit Polyclonal anti-MPL antibody is suitable to detect MPL in samples from Human. It has been validated for FACS.

Quick Overview for MPL antibody (AA 26-595) (Fluoro550) (ABIN8012514)

Target: MPL (Myeloproliferative Leukemia Virus Oncogene (MPL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MPL antibody is conjugated to Fluoro550

Application: Flow Cytometry (FACS)

Product Details anti-MPL Antibody (Fluoro550)

Binding Specificity: AA 26-595

Purpose: Anti-TPOR/MPL Antibody Fluoro550 Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human TPOR,MPL shares 79.6% amino acid (aa) sequence identity with mouse TPOR,MPL.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TPOR/MPL recombinant protein (Position: Q26-Q595). Human TPOR/MPL shares 79.6% amino acid (aa) sequence identity with mouse TPOR/MPL.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for MPL

Target: MPL (Myeloproliferative Leukemia Virus Oncogene (MPL))

Alternative Name: MPL

Background:

Background: In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.

Gene Full Name: MPL proto-oncogene, thrombopoietin receptor

Gene ID: 352

UniProt: P40238

Pathways: JAK-STAT Signaling