NOX1 antibody (Middle Region) (Fluoro594)

Catalog No. ABIN8013727

The Fluoro594-conjugated Rabbit Polyclonal anti-NOX1 antibody (ABIN8013727) specifically detects NOX1 in FACS. The antibody is reactive with Rat and Mouse samples.

Quick Overview for NOX1 antibody (Middle Region) (Fluoro594) (ABIN8013727)

Target: NOX1 (NADPH Oxidase 1 (NOX1))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOX1 antibody is conjugated to Fluoro594

Application: Flow Cytometry (FACS)

Product Details anti-NOX1 Antibody (Fluoro594)

Binding Specificity: Middle Region

Purpose: Anti-NADPH oxidase 1 NOX1 Antibody Fluoro594 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse sequence by one amino acid.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of rat NOX1, different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for NOX1

Target: NOX1 (NADPH Oxidase 1 (NOX1))

Alternative Name: NOX1

Background:

Background: NOX1 (NADPH OXIDASE 1), also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. The NOX1 gene is mapped to Xq22.1. NOX1 was expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58 % identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of MOX1 in NIH 3T3 cells increased superoxide generation and cell growth. Cells expressing MOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50 % survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion.

Gene Full Name: NADPH oxidase 1

Sequence Similarities: Contains 1 FAD-binding FR-type domain.

Gene ID: 114243

UniProt: Q9WV87

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Proton Transport