NOX5 antibody (C-Term)

Catalog No. ABIN8013799

The Rabbit Polyclonal anti-NOX5 antibody (ABIN8013799) specifically detects NOX5 in WB, IHC, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for NOX5 antibody (C-Term) (ABIN8013799)

Target: NOX5 (NADPH Oxidase 5 (NOX5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOX5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-NOX5 Antibody

Binding Specificity: C-Term

Purpose: Anti-NADPH oxidase 5 NOX5 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NADPH oxidase 5 NOX5 Antibody. Tested in IHC, ICC/IF, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human NOX5.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NOX5

Target: NOX5 (NADPH Oxidase 5 (NOX5))

Alternative Name: NOX5

Background:

Background: NOX5 (Nadph Oxidase 5), also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. The International Radiation Hybrid Mapping Consortium mapped the NOX5 gene to chromosome 15. NOX5 is a novel NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. Banfi et al. (2001) found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, Kamiguti et al. (2005) identified NOX5 as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells (HC), but not normal B cells.

Gene Full Name: NADPH oxidase 5

Sequence Similarities: Contains 4 EF-hand domains.

Molecular Weight: 86 kDa

UniProt: Q96PH1

Pathways: Proton Transport