NUB1 antibody (AA 190-543) (Biotin)

Catalog No. ABIN8014174

The Biotin-conjugated Rabbit Polyclonal anti-NUB1 antibody (ABIN8014174) specifically detects NUB1 in WB, IHC and ELISA. The antibody is reactive with Human, Mouse, Monkey and Rat samples.

Quick Overview for NUB1 antibody (AA 190-543) (Biotin) (ABIN8014174)

Target: NUB1 (Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1))

Reactivity: Human, Mouse, Monkey, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUB1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-NUB1 Antibody (Biotin)

Binding Specificity: AA 190-543

Purpose: Anti-NUB1 Antibody Biotin Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NUB1 recombinant protein (Position: K190-D543).

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NUB1

Target: NUB1 (Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1))

Alternative Name: NUB1

Background:

Background: NEDD8 µLtimate buster 1 is a protein that in humans is encoded by the NUB1 gene. This gene encodes a protein that functions as a negative regulator of NEDD8, a ubiquitin-like protein that conjugates with cullin family members in order to regulate vital biological events. The protein encoded by this gene regulates the NEDD8 conjugation system post-transcriptionally by recruiting NEDD8 and its conjugates to the proteasome for degradation. This protein interacts with the product of the AIPL1 gene, which is associated with Leber congenital amaurosis, an inherited retinopathy, and mutations in that gene can abolish interaction with this protein, which may contribute to the pathogenesis. This protein is also known to accumulate in Lewy bodies in Parkinson's disease and dementia with Lewy bodies, and in glial cytoplasmic inclusions in multiple system atrophy, with this abnormal accumulation being specific to alpha-synucleinopathy lesions. Alternative splicing of this gene results in multiple transcript variants.

Gene Full Name: negative regulator of ubiquitin like proteins 1

Gene ID: 51667

UniProt: Q9Y5A7