NUB1 antibody (AA 190-543)

Catalog No. ABIN8014184

This Rabbit Polyclonal antibody specifically detects NUB1 in WB, IHC, ELISA, FACS, IF and ICC. It exhibits reactivity toward Human, Mouse, Monkey and Rat.

Quick Overview for NUB1 antibody (AA 190-543) (ABIN8014184)

Target: NUB1 (Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1))

Reactivity: Human, Mouse, Monkey, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-NUB1 Antibody

Binding Specificity: AA 190-543

Purpose: Anti-NUB1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NUB1 Antibody. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NUB1 recombinant protein (Position: K190-D543).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NUB1

Target: NUB1 (Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1))

Alternative Name: NUB1

Background:

Background: NEDD8 µLtimate buster 1 is a protein that in humans is encoded by the NUB1 gene. This gene encodes a protein that functions as a negative regulator of NEDD8, a ubiquitin-like protein that conjugates with cullin family members in order to regulate vital biological events. The protein encoded by this gene regulates the NEDD8 conjugation system post-transcriptionally by recruiting NEDD8 and its conjugates to the proteasome for degradation. This protein interacts with the product of the AIPL1 gene, which is associated with Leber congenital amaurosis, an inherited retinopathy, and mutations in that gene can abolish interaction with this protein, which may contribute to the pathogenesis. This protein is also known to accumulate in Lewy bodies in Parkinson's disease and dementia with Lewy bodies, and in glial cytoplasmic inclusions in multiple system atrophy, with this abnormal accumulation being specific to alpha-synucleinopathy lesions. Alternative splicing of this gene results in multiple transcript variants.

Gene Full Name: negative regulator of ubiquitin like proteins 1

Molecular Weight: 75 kDa

Gene ID: 51667

UniProt: Q9Y5A7