NFKBIB antibody (AA 56-237)

Catalog No. ABIN8015306

This Rabbit Polyclonal antibody specifically detects NFKBIB in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NFKBIB antibody (AA 56-237) (ABIN8015306)

Target: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKBIB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-NFKBIB Antibody

Binding Specificity: AA 56-237

Purpose: Anti-IKB beta/NFKBIB Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human IKB beta shares 82%,80% amino acid (aa) sequence identity with mouse,rat IKB beta,respectively.

Characteristics: Anti-IKB beta/NFKBIB Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IKB beta recombinant protein (Position: E56-E237). Human IKB beta shares 82% and 80% amino acid (aa) sequence identity with mouse and rat IKB beta, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NFKBIB

Target: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Alternative Name: NFKBIB

Background:

Background: NF-kappa-B inhibitor beta, also known as IKBB or TRIP9, is a protein that in humans is encoded by the NFKBIB gene. The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. This gene is mapped to 19q13.2. It has been found that in vivo, NFKBIB serves both to inhibit and to facilitate the inflammatory response. NFKBIB degradation releases NF-kappa-B dimers, which upregulate proinflammatory target genes such as TNF-alpha. Surprisingly, absence of NFKBIB results in a dramatic reduction of TNF-alpha in response to lipopolysaccharide, even though activation of NF-kappa-B is normal.

Gene Full Name: NFKB inhibitor beta

Sequence Similarities: Belongs to the NF-kappa-B inhibitor family.

Molecular Weight: 45-48 kDa

Gene ID: 4793

UniProt: Q15653

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Maintenance of Protein Location, Toll-Like Receptors Cascades