NFS1 antibody (AA 107-356)

Catalog No. ABIN8015328

The Rabbit Polyclonal anti-NFS1 antibody is suitable to detect NFS1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, FACS and IP.

Quick Overview for NFS1 antibody (AA 107-356) (ABIN8015328)

Target: NFS1 (NFS1, Cysteine Desulfurase (NFS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Grade: Carrier-free

Product Details anti-NFS1 Antibody

Binding Specificity: AA 107-356

Purpose: Anti-NFS1 Antibody

Predicted Reactivity: Human NFS1 shares 96.4%,96% amino acid (aa) sequence identity with mouse,rat NFS1,respectively.

Characteristics: Anti-NFS1 Antibody. Tested in WB, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NFS1 recombinant protein (Position: R107-L356). Human NFS1 shares 96.4% and 96% amino acid (aa) sequence identity with mouse and rat NFS1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NFS1

Target: NFS1 (NFS1, Cysteine Desulfurase (NFS1))

Alternative Name: NFS1

Background:

Background: Cysteine desulfurase, mitochondrial is an enzyme that in humans is encoded by the NFS1 gene. Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH . The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described.

Gene Full Name: NFS1 cysteine desulfurase

Molecular Weight: 45 kDa

Gene ID: 9054

UniProt: Q9Y697

Pathways: Transition Metal Ion Homeostasis