Nucleolin antibody (AA 219-629)

Catalog No. ABIN8017210

The Rabbit Polyclonal anti-Nucleolin antibody (ABIN8017210) specifically detects Nucleolin in WB, IHC, IF, FACS, ICC and ELISA. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for Nucleolin antibody (AA 219-629) (ABIN8017210)

Target: Nucleolin (NCL)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), ELISA

Grade: Carrier-free

Product Details anti-Nucleolin Antibody

Binding Specificity: AA 219-629

Purpose: Anti-Nucleolin/NCL Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Nucleolin/NCL Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Nucleolin/NCL recombinant protein (Position: K219-A629).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/mL Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: NCL

Background:

Background: Nucleolin is a protein that in humans is encoded by the NCL gene. It is mapped to 2q37.1. Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

Gene Full Name: nucleolin

Molecular Weight: 100-110 kDa

Gene ID: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization