NUMB antibody (AA 59-567)

Catalog No. ABIN8017794

The Rabbit Polyclonal anti-NUMB antibody is suitable to detect NUMB in samples from Human, Mouse, Rat and Monkey. It has been validated for WB, ELISA and FACS.

Quick Overview for NUMB antibody (AA 59-567) (ABIN8017794)

Target: NUMB (Numb Homolog (NUMB))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUMB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-NUMB Antibody

Binding Specificity: AA 59-567

Purpose: Anti-NUMB Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human NUMB shares 93.7% amino acid (aa) sequence identity with both mouse,rat NUMB.

Characteristics: Anti-NUMB Antibody. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NUMB recombinant protein (Position: E59-A567). Human NUMB shares 93.7% amino acid (aa) sequence identity with both mouse and rat NUMB.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Monkey, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NUMB

Target: NUMB (Numb Homolog (NUMB))

Alternative Name: NUMB

Background:

Background: Protein numb homolog is a protein that in humans is encoded by the NUMB gene. It is mapped to 14q24.2 to q24.3. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The primary function of Numb in cell differentiation is as an inhibitor of Notch signaling which is essential for maintaining self-renewal potential in stem and progenitor cells. Numb also plays a crucial role in asymmetrical cell division during development, allowing for differential cell fate specification in the central and peripheral nervous systems. What's more, the numb gene protein product controls binary cell fate decisions in the peripheral and central nervous systems of both invertebrates and mammals during neurogenesis.

Gene Full Name: NUMB endocytic adaptor protein

Molecular Weight: 72-75 kDa

Gene ID: 8650

UniProt: P49757

Pathways: Cell-Cell Junction Organization