Occludin antibody (AA 373-522) (Fluoro488)

Catalog No. ABIN8017863

The Rabbit Polyclonal anti-Occludin antibody is suitable to detect Occludin in samples from Human. It has been validated for FACS.

Quick Overview for Occludin antibody (AA 373-522) (Fluoro488) (ABIN8017863)

Target: Occludin (OCLN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Occludin antibody is conjugated to Fluoro488

Application: Flow Cytometry (FACS)

Product Details anti-Occludin Antibody (Fluoro488)

Binding Specificity: AA 373-522

Purpose: Anti-Occludin/OCLN Antibody Fluoro488 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human Occludin shares 89% amino acid (aa) sequence identity with both mouse,rat Occludin.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Occludin recombinant protein (Position: N373-T522). Human Occludin shares 89% amino acid (aa) sequence identity with both mouse and rat Occludin.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: OCLN

Background:

Background: Occludin is an integral membrane protein that is located at tight junctions. It is a member of a family of proteins containing the highly conserved Marvel domain, which contains 4 transmembrane-helix regions. This gene is mapped to 5q13.2. Occludin regulates TGF-beta receptor type I localization for efficient TGF-beta-dependent dissolution of tight junctions during epithelial-mesenchymal transitions. Human Occludin is an essential hepatitis C virus (HCV) cell entry factor that is able to render murine cells infectable with HCV glycoproteins. It has been found that occludin is involved in cell migration and functions to recruit active PI3 kinase to the leading edge, resulting in RAC1 activation and formation of lamellipodia.

Gene Full Name: occludin

Sequence Similarities: Belongs to the ELL/occludin family.

Gene ID: 100506658

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C