Oncostatin M antibody (AA 26-220)

Catalog No. ABIN8018058

The Rabbit Polyclonal anti-Oncostatin M antibody is suitable to detect Oncostatin M in samples from Human. It has been validated for WB, ELISA and IHC.

Quick Overview for Oncostatin M antibody (AA 26-220) (ABIN8018058)

Target: Oncostatin M (OSM)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Oncostatin M antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-Oncostatin M Antibody

Binding Specificity: AA 26-220

Purpose: Anti-Oncostatin M/OSM Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: coli-derived human Oncostatin M recombinant protein (Position: A26-R220). Human Oncostatin M shares 47.1%,47.6% amino acid (aa) sequence identity with mouse,rat Oncostatin M,respectively.

Characteristics: Anti-Oncostatin M/OSM Antibody. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Oncostatin M recombinant protein (Position: A26-R220). Human Oncostatin M shares 47.1% and 47.6% amino acid (aa) sequence identity with mouse and rat Oncostatin M, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human ELISA, 0.1-0.5 μg/mL, -Western blot, 0.1-0.5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Oncostatin M

Target: Oncostatin M (OSM)

Alternative Name: OSM

Background:

Background: OSM (ONCOSTATIN M) is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. OSM is mapped on 22q12.2. It has the ability to inhibit the growth of human A375 melanoma cells but not normal human fibroblasts. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM also has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells, a function shared by LIF, CSF3, and IL6. The ion of gene transcription was telomeric to centromeric, with the OSM gene located upstream of the LIF gene.

Gene Full Name: oncostatin M

Molecular Weight: 40 kDa

Gene ID: 5008

UniProt: P13725

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion