ORC1 antibody (AA 628-861) (HRP)

Catalog No. ABIN8018386

The HRP-conjugated Rabbit Polyclonal anti-ORC1 antibody (ABIN8018386) specifically detects ORC1 in WB, ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for ORC1 antibody (AA 628-861) (HRP) (ABIN8018386)

Target: ORC1 (ORC1L) (Origin Recognition Complex, Subunit 1 (ORC1L))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ORC1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-ORC1 Antibody (HRP)

Binding Specificity: AA 628-861

Purpose: Anti-ORC1 Antibody HRP Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ORC1 recombinant protein (Position: H628-E861).

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ORC1

Target: ORC1 (ORC1L) (Origin Recognition Complex, Subunit 1 (ORC1L))

Alternative Name: ORC1

Background:

Background: Origin recognition complex subunit 1 is a protein that in humans is encoded by the ORC1 gene. The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: origin recognition complex subunit 1

Gene ID: 4998

UniProt: Q13415

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA