ORC5 antibody (Middle Region)

Catalog No. ABIN8018401

The Rabbit Polyclonal anti-ORC5 antibody (ABIN8018401) specifically detects ORC5 in WB, FACS, IF and ICC. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for ORC5 antibody (Middle Region) (ABIN8018401)

Target: ORC5 (Origin Recognition Complex, Subunit 5 (ORC5))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ORC5 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-ORC5 Antibody

Binding Specificity: Middle Region

Purpose: Anti-ORC5L/ORC5 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: identical to the related mouse sequence.

Characteristics: Anti-ORC5L/ORC5 Antibody. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ORC5, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ORC5

Target: ORC5 (Origin Recognition Complex, Subunit 5 (ORC5))

Alternative Name: ORC5

Background:

Background: Origin recognition complex subunit 5 is a protein that in humans is encoded by the ORC5 (ORC5L) gene. The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Gene Full Name: origin recognition complex subunit 5

Molecular Weight: 50 kDa

Gene ID: 5001

UniProt: O43913

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA