PELI2 antibody (AA 118-420)

Catalog No. ABIN8020027

The Rabbit Polyclonal anti-PELI2 antibody (ABIN8020027) specifically detects PELI2 in WB, ELISA, FACS, IHC, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PELI2 antibody (AA 118-420) (ABIN8020027)

Target: PELI2 (Pellino Homolog 2 (Drosophila) (PELI2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PELI2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-PELI2 Antibody

Binding Specificity: AA 118-420

Purpose: Anti-PELI2 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human PELI2 shares 97.4% amino acid (aa) sequence identity with mouse?PELI2.

Characteristics: Anti-PELI2 Antibody. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PELI2 recombinant protein (Position: D118-D420). Human PELI2 shares 97.4% amino acid (aa) sequence identity with mouse?PELI2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PELI2

Target: PELI2 (Pellino Homolog 2 (Drosophila) (PELI2))

Alternative Name: PELI2

Background:

Background: Protein pellino homolog 2 is a protein that in humans is encoded by the PELI2 gene. PELI2 is a E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. It is involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. It mediates IL1B-induced IRAK1 'Lys-63'-linked polyubiquitination and possibly 'Lys-48'-linked ubiquitination. It may be important for LPS- and IL1B-induced MAP3K7- dependent, but not MAP3K3-dependent, NF-kappa-B activation. And it can activate the MAP (mitogen activated protein) kinase pathway leading to activation of ELK1.

Gene Full Name: pellino E3 ubiquitin protein ligase family member 2

Molecular Weight: 50 kDa

Gene ID: 57161

UniProt: Q9HAT8

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades