PEPD antibody (AA 33-493)

Catalog No. ABIN8020127

This Rabbit Polyclonal antibody specifically detects PEPD in WB, ELISA, FACS, ICC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PEPD antibody (AA 33-493) (ABIN8020127)

Target: PEPD (Peptidase D (PEPD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEPD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-PEPD Antibody

Binding Specificity: AA 33-493

Purpose: Anti-PEPD Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human PEPD shares 89.4% amino acid (aa) sequence identity with mouse,rat PEPD.

Characteristics: Anti-PEPD Antibody. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PEPD recombinant protein (Position: R33-K493). Human PEPD shares 89.4% amino acid (aa) sequence identity with mouse and rat PEPD.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PEPD

Target: PEPD (Peptidase D (PEPD))

Alternative Name: PEPD

Background:

Background: Xaa-Pro dipeptidase, also known as prolidase, is an enzyme that in humans is encoded by the PEPD gene. This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: peptidase D

Molecular Weight: 60 kDa

Gene ID: 5184

UniProt: P12955