PIN1 antibody (AA 1-163)

Catalog No. ABIN8020283

This Rabbit Polyclonal antibody specifically detects PIN1 in WB, IHC, IF, ICC and FACS. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for PIN1 antibody (AA 1-163) (ABIN8020283)

Target: PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-PIN1 Antibody

Binding Specificity: AA 1-163

Purpose: Anti-Pin1 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human Pin1 shares 95% amino acid (aa) sequence identity with mouse Pin1.

Characteristics: Anti-Pin1 Antibody. Tested in Flow Cytometry, ICC/IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Pin1 recombinant protein (Position: M1-E163). Human Pin1 shares 95% amino acid (aa) sequence identity with mouse Pin1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PIN1

Target: PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))

Alternative Name: PIN1

Background:

Background: Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, also called DOD, is an enzyme that in humans is encoded by the PIN1 gene. It is mapped to 19p13.2. The enzyme binds to a subset of proteins and thus plays a role as a post phospho PIN1rol in regulating protein function. Studies have shown that the deregulation of PIN1 may play a pivotal role in various diseases. Notably, the up-regulation of PIN1 may be implicated in certain cancers, and the down-regulation of Pin1 may be implicated in Alzheimer's disease. Inhibitors of PIN1 may have therapeutic implications for cancer and immune disorders. PIN1 activity regulates the outcome of proline-ed kinase (e.g. MAPK, CDK or GSK3) signalling and consequently regulates cell proliferation (in part through control of cyclin D1 levels and stability) and cell survival. PIN1 also has an essential role in maintaining cell proliferation and regulating cyclin D1 function.

Gene Full Name: peptidylprolyl cis/trans isomerase, NIMA-interacting 1

Sequence Similarities: Contains 1 PpiC domain.

Molecular Weight: 18 kDa

Gene ID: 5300

UniProt: Q13526