PDE6A antibody (AA 11-237)

Catalog No. ABIN8021500

The Rabbit Polyclonal anti-PDE6A antibody (ABIN8021500) specifically detects PDE6A in WB, IHC, ELISA, FACS, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PDE6A antibody (AA 11-237) (ABIN8021500)

Target: PDE6A (phosphodiesterase 6A, CGMP-Specific, Rod, alpha (PDE6A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE6A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-PDE6A Antibody

Binding Specificity: AA 11-237

Purpose: Anti-PDE6 alpha/PDE6A Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PDE6 alpha/PDE6A Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PDE6 alpha/PDE6A recombinant protein (Position: K11-R237).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL,Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PDE6A

Target: PDE6A (phosphodiesterase 6A, CGMP-Specific, Rod, alpha (PDE6A))

Alternative Name: PDE6A

Background:

Background: This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa.

Gene Full Name: phosphodiesterase 6A

Molecular Weight: 100 kDa

Gene ID: 5145

UniProt: P16499

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction