PDE6B antibody (AA 25-237)

Catalog No. ABIN8021511

The Rabbit Polyclonal anti-PDE6B antibody is suitable to detect PDE6B in samples from Human, Mouse and Rat. It has been validated for WB, IHC, FACS, ELISA, IF and ICC.

Quick Overview for PDE6B antibody (AA 25-237) (ABIN8021511)

Target: PDE6B (phosphodiesterase 6B, CGMP-Specific, Rod, beta (PDE6B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE6B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-PDE6B Antibody

Binding Specificity: AA 25-237

Purpose: Anti-PDE6 beta/PDE6B Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PDE6 beta/PDE6B Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PDE6 beta/PDE6B recombinant protein (Position: K25-Q237).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PDE6B

Target: PDE6B (phosphodiesterase 6B, CGMP-Specific, Rod, beta (PDE6B))

Alternative Name: PDE6B

Background:

Background: Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE), resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels, and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta, and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: phosphodiesterase 6B

Molecular Weight: 98 kDa

Gene ID: 5158

UniProt: P35913

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction