PYGL antibody (AA 313-804)

Catalog No. ABIN8022433

The Rabbit Polyclonal anti-PYGL antibody (ABIN8022433) specifically detects PYGL in WB, IHC, ELISA and FACS. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for PYGL antibody (AA 313-804) (ABIN8022433)

Target: PYGL (phosphorylase, Glycogen, Liver (PYGL))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYGL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-PYGL Antibody

Binding Specificity: AA 313-804

Purpose: Anti-PYGL Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PYGL Antibody. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PYGL recombinant protein (Position: K313-K804).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PYGL

Target: PYGL (phosphorylase, Glycogen, Liver (PYGL))

Alternative Name: PYGL

Background:

Background: Glycogen phosphorylase, liver form (PYGL), also known as human liver glycogen phosphorylase (HLGP), is an enzyme that in humans is encoded by the PYGL gene on chromosome 14. This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.

Gene Full Name: glycogen phosphorylase L

Molecular Weight: 97 kDa

Gene ID: 5836

UniProt: P06737

Pathways: Carbohydrate Homeostasis, Cellular Glucan Metabolic Process