PNKP antibody (AA 43-487)

Catalog No. ABIN8024396

This Rabbit Polyclonal antibody specifically detects PNKP in WB, ELISA, IF, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PNKP antibody (AA 43-487) (ABIN8024396)

Target: PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PNKP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-PNKP Antibody

Binding Specificity: AA 43-487

Purpose: Anti-PNKP Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human PNKP shares 81.8% amino acid (aa) sequence identity with mouse PNKP.

Characteristics: Anti-PNKP Antibody. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PNKP recombinant protein (Position: D43-E487). Human PNKP shares 81.8% amino acid (aa) sequence identity with mouse PNKP.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PNKP

Target: PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))

Alternative Name: PNKP

Background:

Background: This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.

Gene Full Name: polynucleotide kinase 3'-phosphatase

Molecular Weight: 57 kDa

Gene ID: 11284

UniProt: Q96T60

Pathways: DNA Damage Repair, Nucleotide Phosphorylation