Prohibitin 2 antibody (AA 1-299) (APC)

Catalog No. ABIN8025669

The Rabbit Polyclonal anti-Prohibitin 2 antibody is suitable to detect Prohibitin 2 in samples from Human, Mouse and Rat. It has been validated for FACS.

Quick Overview for Prohibitin 2 antibody (AA 1-299) (APC) (ABIN8025669)

Target: Prohibitin 2 (PHB2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prohibitin 2 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Product Details anti-Prohibitin 2 Antibody (APC)

Binding Specificity: AA 1-299

Purpose: Anti-REA/PHB2 Antibody APC Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human REA/PHB2 recombinant protein (Position: M1-K299).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for Prohibitin 2

Target: Prohibitin 2 (PHB2)

Alternative Name: PHB2

Background:

Background: PHB2 (Prohibitin 2), also called Repressor of Estrogen Receptor Activity (REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the PHB2 gene to chromosome 12. Montano et al. (1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta (ESR2), REA suppressed activation of a < a href=https://www.bosterbio.com/cells/reporter-cell-lines style=color:#ea8d28>reporter gene in a dose-dependent manner. REA had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of REA were required for its repressor activity.

Gene Full Name: prohibitin 2

Gene ID: 11331

UniProt: Q99623

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling