Prohibitin 2 antibody (AA 1-299)

Catalog No. ABIN8025683

This Mouse Monoclonal antibody specifically detects Prohibitin 2 in WB, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Prohibitin 2 antibody (AA 1-299) (ABIN8025683)

Target: Prohibitin 2 (PHB2)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prohibitin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Clone: 2B5

Product Details anti-Prohibitin 2 Antibody

Binding Specificity: AA 1-299

Purpose: Anti-REA/PHB2 Antibody (monoclonal, 2B5)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-REA/PHB2 Antibody.(monoclonal, 2B5). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human REA/PHB2 recombinant protein (Position: M1-K299).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Prohibitin 2

Target: Prohibitin 2 (PHB2)

Alternative Name: PHB2

Background:

Background: PHB2 (Prohibitin 2), also called Repressor of Estrogen Receptor Activity (REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the PHB2 gene to chromosome 12. Montano et al. (1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta (ESR2), REA suppressed activation of a < a href=https://www.bosterbio.com/cells/reporter-cell-lines style=color:#ea8d28>reporter gene in a dose-dependent manner. REA had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of REA were required for its repressor activity.

Gene Full Name: prohibitin 2

Molecular Weight: 32 kDa

Gene ID: 11331

UniProt: Q99623

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling