PCCA antibody (AA 403-728) (Biotin)

Catalog No. ABIN8026137

The Rabbit Polyclonal anti-PCCA antibody is suitable to detect PCCA in samples from Human, Mouse and Rat. It has been validated for WB, IHC and ELISA.

Quick Overview for PCCA antibody (AA 403-728) (Biotin) (ABIN8026137)

Target: PCCA (Propionyl CoA Carboxylase, alpha Polypeptide (PCCA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCCA antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-PCCA Antibody (Biotin)

Binding Specificity: AA 403-728

Purpose: Anti-PCCA Antibody Biotin Conjugated

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human PCCA shares 89.6%,89% amino acid (aa) sequence identity with mouse,rat PCCA,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PCCA recombinant protein (Position: E403-E728). Human PCCA shares 89.6% and 89% amino acid (aa) sequence identity with mouse and rat PCCA, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PCCA

Target: PCCA (Propionyl CoA Carboxylase, alpha Polypeptide (PCCA))

Alternative Name: PCCA

Background:

Background: The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: propionyl-CoA carboxylase subunit alpha

Gene ID: 5095

UniProt: P05165

Pathways: Monocarboxylic Acid Catabolic Process