PCCA antibody (AA 403-728)

Catalog No. ABIN8026147

The Rabbit Polyclonal anti-PCCA antibody (ABIN8026147) specifically detects PCCA in WB, IHC, ELISA and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PCCA antibody (AA 403-728) (ABIN8026147)

Target: PCCA (Propionyl CoA Carboxylase, alpha Polypeptide (PCCA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCCA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-PCCA Antibody

Binding Specificity: AA 403-728

Purpose: Anti-PCCA Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human PCCA shares 89.6%,89% amino acid (aa) sequence identity with mouse,rat PCCA,respectively.

Characteristics: Anti-PCCA Antibody. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PCCA recombinant protein (Position: E403-E728). Human PCCA shares 89.6% and 89% amino acid (aa) sequence identity with mouse and rat PCCA, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PCCA

Target: PCCA (Propionyl CoA Carboxylase, alpha Polypeptide (PCCA))

Alternative Name: PCCA

Background:

Background: The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: propionyl-CoA carboxylase subunit alpha

Molecular Weight: 70 kDa

Gene ID: 5095

UniProt: P05165

Pathways: Monocarboxylic Acid Catabolic Process