PKDCC antibody (AA 129-490)

Catalog No. ABIN8027575

The Rabbit Polyclonal anti-PKDCC antibody (ABIN8027575) specifically detects PKDCC in WB, ELISA, FACS, IHC, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for PKDCC antibody (AA 129-490) (ABIN8027575)

Target: PKDCC (Protein Kinase Domain Containing, Cytoplasmic Homolog (Mouse) (PKDCC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKDCC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-PKDCC Antibody

Binding Specificity: AA 129-490

Purpose: Anti-SGK493/PKDCC Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human PKDCC shares 93.4% amino acid (aa) sequence identity with mouse PKDCC.

Characteristics: Anti-SGK493/PKDCC Antibody. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SGK493/PKDCC recombinant protein (Position: R129-K490). Human PKDCC shares 93.4% amino acid (aa) sequence identity with mouse PKDCC.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PKDCC

Target: PKDCC (Protein Kinase Domain Containing, Cytoplasmic Homolog (Mouse) (PKDCC))

Alternative Name: PKDCC

Background:

Background: VLK was identified as a novel protein kinase that was induced after the differentiation of cultured embryonic stem cells into mesendoderm. It has no homologs in invertebrates, but is highly conserved in vertebrate species although it does not belong to any known protein kinase groups. VLK is initially expressed in E-cadherin-positive anterior visceral endoderm and mesendoderm, but its expression is later confined to E-cadherin-negative mesenchyme. It is enriched in the Golgi apparatus and is thought to regulate the rate of protein export from the Golgi. Targeted disruption of VLK in mice leads to a defect in lung development and neonatal lethality. It has been suggested that mutations in VLK may be associated with the allergic condition atopy.

Gene Full Name: protein kinase domain containing, cytoplasmic

Molecular Weight: 54 kDa

Gene ID: 91461

UniProt: Q504Y2